PMC

Analysis of neonatal and adult wound beds. (A,B) TOPGFP expression in fibroblasts of PW5, PW7 and PW10 wound beds of mice wounded at neonatal (P2) and adult (P21 and P50) stages. Wound beds were immunostained for GFP (green), Lrig1 (red) and Sca1 (blue) (A). TOPGFP expression in the boxed areas is shown at higher magnification beneath. Placodes are marked with asterisks and dotted lines indicate the basement membrane. (B) Quantitation of GFP⁺ cells in wound beds at the indicated time points. n=4 biological replicates, except n=3 for P21 and P50 at PW7. (C) Dermal cells per mm² wound bed area over time in adult and neonatal wounds. n=4 biological replicates. (D,E) Mean immunofluorescence quantification in the neonatal (P2) and adult (P21, P50) wound beds over time for Lrig1 (D) and Sca1 (E). n=4 biological replicates per time point. (F) Dkk1 expression (red) in TOPGFP (green) P2 and P50 wound beds at PW7. (G,H) PW7 wound beds after wounding at the indicated time point, immunostained for Itga8 (green, G) and αSma (green, H). (I) Quantitation of the density of PDGFRaH2BeGFP-expressing cells in PW7 wound beds wounded at the indicated time points. n=3 biological replicates per time point. (J) Quantitation of Ki67⁺ cells in the wound beds after wounding at the indicated time points. n=8 (P2), n=6 (P4), n=4 (P21), n=5 (P50) biological replicates. Nuclei were labelled with DAPI (white in A; blue in F-H). Data shown are means±s.d. ns, not significant; *P<0.05, ***P<0.0005. AU, arbitrary units; IF, immunofluorescence. Scale bars: 100 µm.

Rates of psychiatric beds per 100 000 population (1990-2020). Panel A. Countries with rates lower than 2.4 per 100 000 population at the last data point; Panel B. Countries with rates between 2.4 and 20 per 100 000 population at the last data point; Panel C. Countries with rates higher than 20 per 100 000 population at the last data point;

Rates of psychiatric beds per 100 000 population (1990-2019). a) Countries with rates lower than 54 per 100 000 population (at the last data point); b) Countries with rates higher than 54 per 100 000 population (at the last data point).

Numbers of specialized forensic psychiatric beds per 100 000 population (1990-2019). a) Countries with rates 3,9 per 100 000 population or higher at the last data point; b) Countries with rates lower than 3,9 per 100 000 population at the last data point.